Tumor Specific Gene Regulation by Ginseng Saponin Rh2 in Mice Bearing Lung Cancer

Objective To study gene differential expression profiles by ginsenoside Rh2 in normal and tumor-bearing mice. Methods Gene expression profiles after treatment with Rh2 in normal and tumor-bearing mice were determined by Oligo chip technology as well as the clustering and statistic analysis of single gene.To further validate the microarray results,we used quantitative real-time RT-PCR to examine the effect of Rh2 on individual genes. Results As compared with normal mice,treatment with Rh2 changed gene expression more potently in mice bearing tumor. Thereps no obvious correlation on the gene expression profiles between the normal and tumor2bearing mice. Treatment with Rh2 was found to be effectively resistant abnormal gene expression profiles in some major organs that resulted f rom the local implantation of tumor. Conclusion 　The data showed that there is specific regulation of gene expression profiles related to tumor by Rh2 in mice bearing tumor.