Clonal expansion of TCR Vβ subfamily T cells induced by AML-M_5 cells
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Graphical Abstract
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Abstract
Objective To investigate the restricted usage and clonal expansion of TCR Vβ subfamily T cells induced by AML-M_5 cells. Methods The mononuclear cells(MNC) from donor peripheral blood or cord blood were amplified by mixed lymphocyte and tumor culture(MLTC), induced by AML-M_5 cells from 2 patients respectively. The complementarity determining region 3(CDR3) of TCR β with variable region genes was amplified in T cells from the donor or cord blood before and after MLTC using RT-PCR, to detect the usage feat...
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