Inhibitory Effect of Antisense RNA Targeting survivin Gene Combined with Taxotere on Transplanted Multidrug Resistant Colorectal Carcinoma in Nude Mice

Objective 　To investigate the anti-tumor effect of an antisense RNA eukaryotic expression plasmid pcDNA3. 0/ survivin targeting survivin gene combined with taxotere on multiple drug resistant (MDR) colorectal carcinoma in vivo. Methods 　Human colorectal carcinoma model of nude mice were given the following treatment, taxotere, antisense survivin RNA, and antisense survivin RNA combined with taxotere, respectively. Tumor growth inhibitary rates were calculated when the mice were killed 4 weeks later. The survivin protein expression was detected by immunohistochemistry method and tumor apoptosis was analyzed by TUNEL assay. Results 　Taxotere had no therapeutic effect on transplanted colorectal tumor. Antisense survivin RNA could down-regulate survivin protein expression in tumor tissue, and taxotere didn't have the same effect . When treated with antisense survivin RNA and taxotere, tumor tissue survivin protein decreased most obviously. Apoptosis index ( AI) was ( 7. 0 ±1. 83 ) %, (21. 50 ±2. 38) % and (43. 5 ±3. 87) % respectively in the three group s above. AI in the former group had no statistical significance compared with the control group ( P > 0. 05), and that in the latter two groups were significantly higher than the cont rol group ( P < 0. 01) . Conclusion 　The antisense survivin RNA could enhance the chemosensitivity of colorectal carcinoma to taxotere.