The Effects of Three Antisense c-myc RNA Tranquilization Expression on the Gronth and proliferation of HL-60

Objective To compare the effects of different antisense c-myc molecule on the growth and proliferation of HL-60. Methods Three antisense retrovirus expression vectors for human c-myc exon 1,2 and 3, respectively named aM1, aM2 and aM3, were tranfered into cultured HL-60 cells. Results (1)Stable expression of the three vectors reduced HL-60 Myc expression(aM2>aM1>aM3);(2)aM2 increased the ratio of G0/G1, decreased the proportions of S phase, DNA and total protein synthesis. However, little change ...