Influence on the Oncogenes Expression of Renal Cell Carinoma (RCC) Cells Transduced with Human TNFA Gene
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Graphical Abstract
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Abstract
Objective To study the influence on the oncogenes expression of the human renal cell carcinoma (RCC) 786-0 cells transduced with human TNFα gene. Methods Recombinant retroviral vector pL(TNFα) SN was constructed and transfected into the packaged cell.The confirmed retrovirus were infected to RCC cells,and the biological activity of rhTNFα was tested by MTT method.Then,oncogenes expression and TNFα transduced RCC cells were observed by immunohistochemistry. Results The expression level of bcl 2 was not affec...
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