Constructed Eukaryotic Expression Plasmid of Small Hairpin RNA (shRNA) of PCNA and Investigated the Inhibitory Effect on Hela Cell

Objective Constructed eukaryotic expression plasmid of small hairpin RNA (shRNA) of PCNA, then expressed plasmid in Hela cells and investigated the inhibitory effect of shRNA of PCNA on Hela cell carcinoma cell proliferation. Methods The cDNA shRNA the prime of PCNA was inserted into downstream of the vector pGenesil-1 to obtain the recombinant eukaryotic expression plasmid pGenesil-1-PCNA1-4 which we identified with the methods of enzyme digestion and sequence analysis. The results were consistent with wha...