A Simple Making Method of Tissue Microarray and Validation Study on Immunohistochemistry

Objective 　To search a simple making mothd of tissue microarray and evaluate it s validation. Methods 　A new, simple and easy method was designed to improve the technology of TMA during the experiment . Tissue microarrays with 1. 6 mm in diameter tissue core were const ructed from 124 cases of breast cancer. The tissue microarrays were stained for oest rogen receptor ( ER), progesterone receptor (PR) and c-erBb2 using immunohistochemistry. At the same time the stains on the tissue microarrays were compared with that from the full tissue sections. Results 　A highly significant association was observed between the staining scores (0 ～ 7) obtained f rom the full tissue sections and f rom the tissue microarrays. Concordance for the receptor status (positive / negative) of the whole section and the tissue core were 94. 35 % for ER, 93. 55 % for PR. and 91. 94 % for c-erBb2. The discordance between full section and tissue microarrays was studied using Two 2 Related 2 Samples tests and the result had no statistical significance ( P > 0. 05) . Conclusion 　Tissue microarays with 1. 6 mm in diameter tissue core made by simple method can represent full tissue section on immunohistochemistry study. We believe our method of tissue microarray is a reliable and valid