Combination Therapy for Glioma with HSV-tk/GCV System and Recombinant Human Tumor Necrosis Factor-alpha

Objective To investigate the synergistic anti-glioma effects of HSV-tk/GCV system and Recombinant Human Tumor Necrosis Factor-alpha(rhTNF-α)in vitro. Methods The HSV-tk gene was transferred into C6 cells to obtain C6 tk + cells.The test was divided into four groups:C6 cells group、C6tk/GCV group、C6+rhTNF-α group and C6+tk/GCV+rhTNF-α group.Each group was treated with GCV or/and rhTNF-α.The cells survival rates of every group were determined by MTT technique. Results The tumor cells without any anti-glioma fac...