The mutation and abnormal methylation of VHL tumor-suppressor gene in primary renal cell carcinoma

Objective To evaluate the clinical implications of abnormality of VHL gene in primary renal cell carcinoma(RCC). Methods The mutation and abnormal methylation of VHL gene were detected by Multiplex-PCR, PCR-SSCP in 42 cases primary RCC, 18 cases of normal renal tissues adjacent to neoplas- tic leisons and 10 cases normal renal tissues. Results Mutation of VHL gene was found in 16/28 of Clear RCC, 4/8 of mixed RCC(clear/granular). Abnormal methylation was identified in 6/2...