Eukaryotic Expression of Human Arresten

Objective 　To const ruct the eukaryotic expression of arresten in CHO cell. Methods 　By gene transfected way, pSecTag2-arresten was t ransfected into CHO cell line. The t ransfected cells were grown in DMEM medium containing Zeocin at 72 hours af ter t ransfection, and the positive clone s were selected in Zeocin medium until the 15th day. Expression of arresten mRNA in CHO cell was examined by RT-PCR, the expression of arresten protein was examined by Western-Blot . Results 　Arresten gene was expressed successfully in transfected CHO cell. Conclusion 　The positive CHO cell clones expressing human arresten gene stable obtained, which may be a promising cell model for studying the biological function of the arresten gene and role of arresten in the angiogenesis.