Effect of Vitamin E Succinate on Sensitivity to Tamoxifen in MCF-7 Breast Cancer Cells

Objective To investigate the function of vitamin E succinate on the sensitivity of MCF-7 human breast cancer cells to tamoxifen. Methods MCF-7 human breast cancer cells were treated with VES for 24h at the concentrations of 5μg/ml and 10μg/ml. Then tamoxifen was administrated at the concentrations of 1μM, 2.5 μM, 5μM and 10μM for 24h. The inhibitory effect was measured with MTT method. RT-PCR was applied to detect the transcript of HER-2 gene. Product of HER-2, c-erbB-2 protein expression in cancer cells wa...