Study on Sodium Butyrate Induced Apoptosis in Ovarian Epithelial Carcinoma Cells 3AO

Objective To investigate the mechanism of sodium butyrate (NaB) induced apoptosis in ovarian carcinoma cells 3AO. Methods Human ovarian epithelial cancer 3AO cells were cultured in vitro and treated with NaB of different concentration for different time. Light microscopy and DNA ladder were applied to detect apoptosis. Then the expression of Fas/FasL and bcl-2/Bax,as well as the mitochondrion transmembrane potentials were analyzed through Flow Cytometry. Electron microscopy was performed to observe the cha...