Application of Median-effect Principle in Guantitative Analysis of Antitumor Agents in Vitro

Objective Synergistic and antagonistic effect of combined antitumor agents were analyzed quantitatively with median-effect principle in vitro. Methods The median-effect principle and MTT method were used in HL-60. Results Vincristine and cytarabinum cytotoxic activity of the individual and combined drugs enhanced as drug concentration increased. The sequence of administration did not influence the cytotoxic activity of the combined antitumor drugs. The ratio of drug concentration was a factor to influenc...