人胎盘GST-π抗体的免疫组织化学检测对胆囊癌早期诊断的意义

Value of Immunhistochemical Investigatinn of Anti-GST-π Antibody in the Early Diagsis of the Gallbladder Carcinom

摘要: 应用人胎盘型谷胱甘肽S—转移酶（GST-π）抗体的ABC法免疫组织化学技术，检测了100例胆囊良、恶性病变组织的GST-π活性，结果表明，22例正常胆囊粘膜对照组GST-π阳性率为9%，12例胆囊炎和4例胆囊腺瘤GST-π阳性率分别为16.67%和25%，视为癌前病变的异型增生组，GST-π阳性率达100%，随着异型增生程度加重，GST-π活性增强。38例胆囊腺癌GST-π阳性率为87%，其中高分化和中分化型腺癌组织中GST-π阳性率为95%，低分化型为100%，未分化型腺癌GST-π阳性率75%，胆囊腺癌、异型增生组GST-π阳性率及平均强度都显著高于良性病变和正常对照组（P

Abstract: Human placental form of giutathione S-transferase (GST-π) was detected in38 gallbladder carcinoma and 24 atypical hyperplasis by Avindin biotin peroxidase complex (ABC) method using anti-GST-π antibody. of 22 normal gallbladder mucosa, 91% were negating for GST-π atain, 95% of 21 well and moderately differentialed gallbladder adenocarcinomas,all of poorly differentiated gallbladder adenocarcinomas, 75% of undifferentiated gallbladder Carcinomas, 100% of atypical hyperplasis were positive for GST-π stain. These results indicate that GST-π is a new sensitive marker for immunohistochmical detection of gallbladder carcinoma.