Abstract: We treat brain tumor with Java brucea Oil-emulsion and eluded pathological micro-struoture changes of tumor cells in clinic.Tumor cells have obvious changes.Glocose of tumor cytoplasm is sparse,organ of cytoplasm is decrease and some was occupied by dense electron substances.The mitochondias are swellen and coagulate dense electron.Substances can be seen in necleus,Whole cell complete denaturation,necrosis,and disappear.