三氧化二砷诱导人肝癌细胞株SMMC-7721凋亡的实验研究

A Preliminary study on arsenic trioxide as an inducer of apoptosis in human hepatocellular carcinoma cell line

摘要: MTT法、AO/EB荧光染色法和流式细胞仪分析法观察As₂O₃对人肝癌细胞林SMMC-7721的作用.结果发现:As₂O₃可显著抑制SMMC-7721细胞的生长;经药物作用后,AO/EB染色时,肝癌细胞在显微镜下呈现典型的凋亡形态学改变;在流式细胞仪上可见亚G_l峰;凋亡呈剂量,时间依赖性特点;As₂O₃使细胞周期阻止于G₂/M期.以上表明:As₂O₃可诱导人肝癌细胞株凋亡,可望为临床应用砷剂治疗肝癌提供实验依据.

Abstract: The effect of arsenic trioxide(As₂O₃)on human hepatocellular carcinoma(HCC)cell line SMMC-7721 was studied by using MTT acridine orange(AO)/ethidiumbromide(EB)flurescent stainning and flow cytometry assays.The results showed that As₂O₃cou-ld significantly inhibit the growth of HCC cells in vitro.After treatment with As₂O₃,the cell's morphologic changes we-re consistent with the characteristics of apoptosis under nurescence microscopy and the sub-G₁ peaks were observed by flow cytome-try assays.It suggested that As₂O₃can induce human HCC cell line SMMC-7721 to undelgo apoptosis,and so As₂O ₃may have a potential value in clinically treating HCC.