Abstract: Aim Doxorubicin(DXR) has been shown to induce DNA strands braking tempoarily and recombination gene deletion and rearrangements and mutation to improve apoptosis we used DXR combined with hepatocyte growth factor(HGF)to hepatic cancer cell line(SMMC-7721). Methods Apoptosis was induced by DXR,Actin D,HGF(10,20,30ng/ml)+DXR(0.0lμg/ml)and physioiogical salt solution and bovine serum albulmin as control.We observed the morphology of apoptosis by H-E and fluency staining. Results At 24h,10%apoptosis and 54%dead cell were seen in large dose DXR gro-up.Apoptosis was less than 5%(P<0.05)in small group of DXR and 30% larger dose group of Actind,respectively.It was found that apopto-sis was increased in a dose-depen-dent manner by DXR(0.01μg/ml)+HGF(10,20,30ng/ml)groups after 48h.When DXR and Actin D were added in turn,It inhibited the effect Of HGF on apoptosis.Conchusion The DXR+HGF may induce apoptosis in hepatic carcinoma cell line and might be related to signal transduction for HGF.