人肝细胞癌及癌周组织内P<sup>53</sup>蛋白表达的研究

人肝细胞癌及癌周组织内P⁵³蛋白表达的研究

摘要: 应用LSAB免疫组化方法检测73例原发性肝细胞癌(HCC))及其癌旁组织中P53蛋白的异常表达。结果显示:P53蛋白在HCC中阳性表达率显著高于癌旁组织, 且与HCC分化程度呈显著正相关，低分化组P53蛋白阳性表达率显著高于高分化组(P<0.05); 癌旁组织中, p53蛋白表达仅见于增生结节中, 肝硬化组织内未见p53蛋白阳性表达。提示P53基因突变是HCC的频发事件, 在HCC的发生发展中发挥重要作用; 增生结节是HCC的癌前病变。

Abstract: The expression of P53 protein was examined in 73 cases of hepatocellular carcinoma (HCC) and its surrounding tissues with LSAB immunohistochemical technique. The results showed that positive expression of P⁵³ in HCC was significantly higher than that in surrounding tissues, and correlated with histological differentation,P⁵³ positive rate in poor differentation was higher than that in well differentation (P＜0.05 ). In surroun-d-ing tissues, P⁵³ protein expression was only in liver hyperplastic nodule(LHN), not in liver cirrhosis(LC). Suggesting that mutation of P⁵³ gene is frequent and correlated with progression of HCC; LHN is preneoplasia lesions of HCC.