β-榄香烯对小鼠B<sub>16</sub>黑色素瘤细胞粘附、运动和间隙连接通讯功能的影响

β-榄香烯对小鼠B₁₆黑色素瘤细胞粘附、运动和间隙连接通讯功能的影响

摘要: 目的：探讨β揽香烯对小鼠B₁₆黑色素瘤细胞粘附和运动能力的影响。方法：采用细胞培养、电镜及划痕染料示踪技术，研究了β榄香烯对小鼠B₁₆黑色素瘤细胞粘附与运动能力、间隙连接结构及其间隙连接通讯功能的影响。结果：20~40ug/ml的β榄香烯可显著抑制B₁₆细胞对纤维结合蛋白和层粘连蛋白两种基质的粘附，抑制B₁₆细胞的运动，延迟吸管尖部所致细胞单层损伤的愈合。结论：提示β榄香烯对B₁₆细胞粘附和运动能力的抑制可能是其抗肿瘤转移作用的机制之一。

Abstract: The influences of β elemene on the abilities of adhesion and motility and on the gap junctional intercellular communication(GJIC) of mice melanoma B₁₆ cells were studied in vitro.It was found that β elemene in 20～40 μg/ml inhibited significantly the adhesion of B ₁₆ cells to fibronectin and lamin,and that the drug in 10～40 μg/ml suppressed the movement of B₁₆ cells in vitro and delayed healing of cell monolayer damage.No obvious effect of β elemene in above concentration on gap junction structure and GJIC of B₁₆ cell was observed.It is suggested that the suppression of β elemene on the adhesion and motility of B₁₆ cells may be an important mechanism of the drug inhibiting tumor metastasis.