端粒酶抑制剂联合放疗对小鼠胃癌的作用研究

Effects of telomerase inhibitor combined with radiotherapy on gastric cancer in mice

摘要: 目的　探讨端粒酶抑制剂对动物在体肿瘤的治疗作用及端粒酶抑制剂对放射治疗的增敏价值。方法　将SGC 790 1胃癌细胞接种BALB/c小鼠, 采用端粒酶抑制剂AZT联合放射治疗小鼠胃癌, 观察其对肿瘤体积和肿瘤端粒酶活性的影响。结果　未治疗组小鼠肿瘤体积增加了 3.3倍, 而AZT、放疗及AZT联合放疗组肿瘤体积分别缩小了 2 3.7%、4 8.8%及 86 .9%。肿瘤端粒酶活性检测, 未治疗组、AZT、放疗及AZT联合放疗组的相对端粒酶活性分别为 10 0, 5 2 .6, 2 3.3及 10 .4。结论　AZT、放疗均能使肿瘤体积缩小并降低端粒酶活性, 且AZT能增加肿瘤的放射敏感性。

Abstract: Objective To investigate the treatment effects of telomerase inhibitor for animal tumor in vivo and the radiosensitive value of telomerase inhibitor. Methods The telomerase inhibitor AZT and radiotherapy were used to treat gastric cancers(SGC 7901) xenograft in BALB/c mice .The tumor was weekly measured and telomerase activity was examined by Semi TRAP assy. Results The tumor size of mice increased 3.3 times in untreated group. AZT, radiotherapy and AZT combined with radiotherapy reduced tumor volume o...