三氧化二砷对人肝癌细胞增殖和超微结构的影响

Effectof Arsenic Trioxideonthe Multiplication and Ultrastructure of Human Hepatocarcinoma Cell Lines

摘要: 目的　明确三氧化二砷 (As2O3 )对人肝癌细胞生长和超微结构的影响。方法　采用活细胞观察法、台盘兰拒染法、MTT法和透射与扫描电镜观察了As2 O3 对体外培养的人肝癌细胞株QGY 770 1和QGY 770 3的生长活力和超微结构的影响。结果　As2 O3 能显著地抑制人肝癌细胞QGY 770 1和QGY 770 3的生长, 用药后诱导其出现了典型的细胞凋亡超微结构改变。结论　As2 O3 对人肝癌细胞的生长有显著抑制作用, 该抑制作用与诱导细胞凋亡密切相关。

Abstract: Objective To study the effect of arsenic trioxide(As 2O 3)on the multiplication and ultrastructure of human hepatocarcinoma cells. Methods As 2O 3 s action on the cell growth and ultrastructure of two human hepatocarcinoma cell lines QGY-7701 and QGY-7703 in vitro was observed by observing living cells, Trypan blue, MTT assay and transmission and scan electronmicroscopy detection. Results As 2O 3 could obviously inhibit the growth of human hepatocarcinoma cells QGY-7701 and QGY-7703 and induce them...