p53与HPV16、18型感染在肺鳞癌中的相关研究

Relationship of human papillomavirus types 16 and 18 infection and p53 gene mutation in lung squamous cell carcinoma

摘要: 目的　探讨HPV与p5 3基因突变在肺鳞癌发病中的相互作用。方法　肺癌组织标本的p5 3基因序列 5～ 8外显子突变分析应用单链构象多态性分析多聚酶链反应EB染色法 (PCR SSCP EB), HPV1 6、1 8型DNA相关序列的检测应用PCR法。结果　 40例肺鳞癌组织标本p5 3基因突变率为 67.5 %, 其中外显子 5～ 8突变率分别为 1 7.5 %、1 2 .5 %、1 7.5 %、2 0 %。肺鳞癌组织中HPV1 6、1 8DNA相关序列的检出率为 42 .5 %。p5 3基因突变与HPVDNA序列的检出之间呈显著负相关。结论　p5 3基因突变与HPV感染在肺鳞癌的发病过程中可能存在相互促进作用。

Abstract: Objective To study the relationship of human papillomavirus (HPV) and p53 gene mutation in the pathogenesis of lung squamous cell carcinoma. Methods PCR-SSCP-EB method was used to detected the mutation of exons 5 to 8 of p53 gene,and the HPV types 16,18 DNA related sequences were detected by PCR. Results 67.5% of 40 tumor tissue specimens had p53 gene mutations. The incidences of mutation of exons 5 to 8 were 17.5%,12.5%,17.5% and 20%,respectively. 42.5% of lung squamous cell carcinoma samples were shown...