腺病毒介导的p27kip1cDNA对胃癌细胞增殖的影响

Effects of adenovirus mediated human p27kip1 antioncogene on the proliferation in gastric cancer cell lines SGC-7901

摘要: 目的　研究 p2 7基因对人胃癌细胞SGC 790 1的抗增殖作用。方法　构建重组体腺病毒Ad p2 7kip1并转染SGC 790 1细胞, 绘制细胞生长曲线, 克隆形成实验, 免疫组化检测PCNA的表达情况。结果　成功构建Ad p2 7kip1, 病毒滴度为 1 .2 4× 1 0 12 pfu/ml,在MOI≥ 5 0时, 即可达到 1 0 0 %的转导效率。在SGC 790 1细胞中表达, 能抑制其生长和集落形成, Ad p2 7kip1和对照组PCNA标记指数分别为 3 5 .2 %及78.6% (P <0 .0 1 )。结论　p2 7可有效抑制胃癌细胞的增殖活性, 这为采用 p2 7kip1cDNA基因治疗胃癌奠定了基础。

Abstract: Objective To study the effects of p27kip1gene on the proliferation in gastric cancer cell lines SGC-7901. Methods A replication-deficient adenovirus vector encoding p27kip1(ad-p27kip1) was constructed and human gastriccancer cell lines SGC-7901were transduced in vitro. Analyse the growth curve,colne formation experiment, and examine the PCNA by immunohistochemistry. Results Ad-p27kip1 containing the p27kip1 sequence was successfully constructed and the virus titer was 1.24×10 12 pfu/ml.The transd...