丙型肝炎病毒NS4B对肝细胞c-myc和ras蛋白表达的影响

The Effect of HCV NS4B on Expressions of c-myc Protein and ras Protein in Hepatic Cells

摘要: 目的研究丙型肝炎病毒非结构蛋白4B(NS4B)对肝细胞内癌基因c-myc和ras蛋白表达的影响,从而探讨其在肝癌发生机制中的可能作用。方法通过脂质体介导法,将空白载体PXCN2及丙型肝炎病毒NS4B重组质粒PCXN2-NS4B引入Chang肝细胞内,并G418筛选作稳定传代,RT-PCR法鉴定质粒成功转染入肝细胞内,免疫细胞化学方法观察细胞内c-myc和ras表达情况。结果获得具有G418抗性的Chang肝细胞;空白对照组及空白载体组无c-myc表达,转染NS4B组c-myc表达率为(21.3±1.2)%,与空白对照组及空白载体组比较差异有显著性;空白对照组及空白载体组ras弱阳性表达,转染NS4B组ras呈阳性表达,与空白对照组及空白载体组比较差异有显著性。结论丙型肝炎病毒NS4B可促进癌基因c-myc和ras表达,并可能在丙型肝炎病毒的致癌机制中起重要作用。

Abstract: Objective To investigate the effect of hepatitis C virus non-structural protein 4B(HCV NS4B)on c-myc and ras gene expressions in hepatic cells,and to study the possible role in the carcinogenesis of hepatoma. Methods The experiment was divided into negative control、empty vector PCXN2 and PCXN2-NS4B.The recombinant plasmid (PCXN2-NS4B) and the empty vector were transfected into Chang liver cells with liposome.Screening was performed with G418.HCV NS4B mRNA was detected by reversetranscription PCR.The protein ...