放射与抗VEGF抗体对人肝癌移植瘤生长的抑制作用
The Inhibiting Effect of Radiation and Anti-VEGF Monoclonal Antibody on the Growth of Human Hepatocellular Carcinoma Xenografts
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摘要: 目的观察放射与抗VEGF单抗对人肝癌裸鼠移植瘤生长的抑制作用。方法SMMC-7721肝癌细胞种植于裸鼠后肢皮下,成瘤动物分为放射+抗体组、放射组、抗体组和对照组,单抗每次每只50μg隔日腹腔给药,共6次,放射剂量20Gy一次给予,测肿瘤直径并计算瘤体积。抗体给药结束后2周处死动物,称瘤重,免疫组化法测肿瘤微血管密度。结果放射和抗VEGF单抗明显抑制肿瘤生长,减少肿瘤微血管密度,但两组之间差异无显著性。放射与抗VEGF单抗联合较单纯放射或抗体抑瘤作用显著,三组瘤重抑制率分别为89.2%、75.3%和65.9%,差异有显著性。结论放射结合抗VEGF单抗显著抑制肝癌移植瘤生长,是肝癌综合治疗的有效途径。Abstract: Objective To investigate the inhibiting effects of combining radiotherapy(RT) with anti-vascular endothelial growth factor(VEGF) monoclonal antibody(mAb) therapy on the growth of human SMMC-7721 hepatocellular carcinoma(HCC) xenografts in vivo. Methods HCC cell line was implanted s.c.in mice.All the mice with xenografts were divided into four groups,RT plus mAb group,RT group,mAb group and control group.Anti-VEGF mAb was injected i.p.on alternate days for a total of six injections at a dosage of 50μg each mo...
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