p16基因在膀胱肿瘤细胞凋亡中的作用

Effect of p16 Gene on the Apoptosis of Bladder Carcinoma Cells

摘要: 目的　探讨p16基因与膀胱肿瘤细胞凋亡的关系。方法　应用SABC免疫组织化学法检测了51例膀胱移行细胞癌p16基因的表达，同时用流式细胞仪检测其细胞凋亡情况。结果　p16阳性表达率为47.1%(24/51)，平均凋亡肿瘤细胞百分比为1.5%，p16阳性表达的肿瘤组织凋亡细胞百分比>1.5%的发生率为62.5%，p16阴性表达的肿瘤组织凋亡细胞百分比>1.5%的发生率为25.9%，两者比较有显著性差异(P<0.01)。结论　p16基因的表达与膀胱肿瘤细胞凋亡有关，p16基因可能参与膀胱肿瘤细胞凋亡的正向调节作用。

Abstract: Objective In order to investigate the effect of p16 gene on bladder carcinoma apoptosis. Methods 51 cases bladder carcinomas were studied by immunohistochemical (IHC) technique,while flow cytometry were used for apoptosis test.Result The positive rate of p16 was 47.1%(24/51).The average percentage of apoptosis cells were 1.5%,the percentage of apoptosis cells in p16 positive tumor tissue was 62.5% (more than 1.5%),the percentage of apoptosis cells in p16 negative tumor tissue were 25.9% (more than 1.5%),there were significant difference between positive and negative groups. Conclusion The expression of p16 was related with bladder tumor cell apoptosis,p16 gene may take part in positive-regulation of bladder tumour cell apoptosis.