人外周血细胞毒性CD3-CD56+ NK细胞高效扩增的研究
Study on efficient expansion of human cytotoxic CD3-CD56+NK cells from peripheral blood
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摘要: 目的 探索从人PBMC中高效扩增细胞毒性CD3-CD5 6 + NK细胞的方法。方法 使用干细胞生长培养基 (SCGM )和RPMI 16 4 0培养基, 在抗CD3单抗、IL 2和植物血凝素 (PHA)的作用下从 5例健康成人PBMC中诱导扩增CD3-CD5 6 + NK细胞, 并用MTT法检测其细胞毒活性。结果 只有在使用SCGM为基础培养基时, PBMC经抗CD3单抗、IL 2作用获得大量增殖, 在PHA存在时获得最大增殖(P <0 .0 5 ), 在 14天时扩增 5 1.3± 7.2倍, 含有 (5 2 .4± 7.9) %CD3-CD5 6 + NK细胞和 (14 .2± 4 .0 ) %CD3+ CD5 6 + T细胞 ;在效 /靶比 10∶1时, 对K5 6 2和Raji的杀伤率分别达83.7%和 5 5 .8%。结论 可使用SCGM, 在抗CD3单抗、IL 2和PHA协同作用下大量扩增细胞毒性CD3-CD5 6 + NK细胞, 为应用NK细胞进行肿瘤过继免疫治疗提供了一种简单有效的扩增NK细胞的方法。Abstract: Objective To study the expanding way of human cytotoxic CD3-CD56+ NK cells from peripheral blood mononuclear cells PBMC. Methods PBMCs were cultured in stem cell growth medium SCGM or RPMI 1640 supplemented with monoclonal anti-CD3 antibodies, IL-2 and PHA at varying concentrations. The cytotoxicity of the expanded cells was detected by MTT method. Results After being activated by monoclonal anti-CD3 antibodies and IL-2, PBMCs cultured in SCGM get the obvious expansion, performed the highest prolifer...
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