Survivin在非霍奇金淋巴瘤中的表达及其意义

A study of the Survivin expression in Non-Hodgkin s lymphomas

摘要: 目的　探讨Survivin在NHL中的表达及其与肿瘤细胞凋亡的关系。方法　应用TdT介导的dUTP缺口末端标记 (TUNEL)技术和免疫组织化学方法, 检测 82例NHL和 17例良性淋巴结病变中细胞凋亡和Survivin蛋白的表达水平。结果　Survivin在NHL中的表达为 4 0 .2 % (33/ 82 ), 在良性淋巴结病变中为 11.8% (2 / 17), 二者差异有显著性 (P <0 .0 5 )。中度和高度侵袭性B细胞淋巴瘤中Survivin基因的表达高于惰性淋巴瘤 (P <0 .0 5 ), 但与T细胞淋巴瘤的侵袭性无关 (P >0 .1)。Survivin基因的表达与侵袭性NHL的凋亡指数的下降明显相关 (P <0 .0 0 1)。结论　Survivin的表达可能通过抑制细胞凋亡在NHL的发生发展中发挥了一定的作用。

Abstract: Objective To explore the expression of survivin and its relation with tumor cell apoptosis of non-Hodgkin s lymphomas. Methods TdT-mediated UTP nick end labeling (TUNEL) and immunohistochemistry were used to study cell apoptosis and expression of survivin genes in 82 non-Hodgkin s lymphoma samples and 17 benign lymph node pathological changes. Results Survivin was positive in 33 of 82( 40.2% ) cases of non-Hodgkin s lymphomas, but its expression was detected only in 2 of 17 cases of benign lymph node le...