人参总皂甙诱导人肝癌细胞分化初探

The Preliminary Study of Induced Differentiation of Total Saponins of Panax Ginseng on Human Hepatoma Cell Line

摘要: 目的　探讨人参总皂甙(TSPG)对人肝癌细胞株HepG2的诱导分化作用。方法　以MTT法，光镜，电子显微镜和流式细胞仪分析法观察TSPG对人肝癌细胞生长增殖，形态，超微结构及细胞周期的影响。结果　癌细胞在400ug/ml TSPG处理后，生长受到明显抑制。光镜，电子显微镜表明经TSPG作用细胞形态向正常肝细胞方向逆转。流式细胞仪分析发现TSPG使细胞生长阻滞于S期。结论　从形态及细胞生物学水平提示TSPG可以诱导人肝癌细胞分化。

Abstract: Objective To explore the induced differentiation effect o f total saponins of Panax ginseng(TSPG)on human hepatoma cell line HepG2.M ethods MTT method,light microscopy,electronic microscopy and flow cytomet ry(FCM) were used. Results Cell growth was dose dependently and signi ficantly inhibited by TSPG at the non-toxic but cytostatic concentration of 400 μg/ml.and TSPG changed the morphology of the cell into almost normal hepatocyte s .TSPG arrested the cell cycle of HepG2 cells at the S phase. Conclusion TSPG seemed to induce normal or mature properties of hepatocytes in human hep atoma cell line.