Abstract:
Objective To construct sense and antisense human bone sialoprotein (BSP) eukaryotic expression vector and observe influence of it s expression in human osteosarcoma cell line MG-63 cells about invasion and metastasis of osteosarcoma. Methods An open reading f rame of human BSP was amplified by PCR method and was reconst ructed into the eukaryotic expressive vector p IRES2-EGFP to construct the hBSP sense or antisense expressive vector . The sense and antisense hBSP eukaryotic expression vector was transfected into MG-63 cells with lipofec tamine. The expression of BSP on the cells was measured by Western blotting. Transwell-chamber methodand scarification was used to evaluate influence of BSP expression on osteosarcoma cell invasion and metastasis. Results The sense and antisense hBSP eukaryotic vector was successfully const ructed and t ransfected. Compared with cont rol cells, expression of BSP increased in the cells by sense hBSP eukaryotic vector transfected and invasive power of these cells is enhance, but expression of BSP decreased in the cells by antisense hBSP eukaryotic vector transfected and invasive power of these cells is been inhabited. Conclusion The change of BSP expression may play an important role in osteosarcoma metastasis. The down-regulation of BSP expression can inhibit abilities of osteosarcoma cell invasion and metastasis.