DNA 双链断裂修复蛋白在宫颈癌和CIN组织中的表达

doi:10.3971/j.issn.1000-8578.571

DNA 双链断裂修复蛋白在宫颈癌和CIN组织中的表达

Expression of DNA Double-strand Break Repair Protein in Cervical Carcinoma and CIN Tissue

摘要

摘要: 目的研究DNA双链断裂修复蛋白（Ku80、DNA-PKcs和ATM）在宫颈癌和宫颈上皮内瘤样病变（CIN）组织中的表达情况,探讨3种蛋白在宫颈癌发生发展中的作用及相互关系。方法应用免疫组化SP法检测41例宫颈癌组织和15例CIN组织中Ku80,DNA-PKcs和ATM蛋白的表达情况。结果 Ku80、DNA-PKcs和ATM蛋白在宫颈癌患者中的阳性率分别为70.7%、68.3%和19.5%,在CIN患者中的阳性率分别为80.0%、73.3%和33.3%;3种蛋白在宫颈癌组织中的表达均低于CIN组织,但差异无统计学意义（P〉0.05）。3种蛋白的表达在不同年龄、病理类型、分化程度和临床分期的宫颈癌患者中差异无统计学意义（P〉0.05）。Spearman等级相关分析,在56例患者中,Ku80与DNA-PKcs的表达呈正相关（r=0.583,P=0.000）;Ku80与ATM的表达亦呈正相关（r=0.274,P=0.041）。结论 3种蛋白可能未在宫颈癌的发生发展中起主要作用;Ku80与DNA-PKcs及与ATM之间存在密切关系。

Abstract: Objective 　To study the expression of DSB repair protein ( including Ku80, DNA-PKcs and ATM) in cervical carcinoma and cervical intra-epithelial neoplasia (CIN), and to explore their roles in neoplasia and tumor progression of cervical carcinoma. Methods 　Immunohistochemistry was used to detect the expressions of Ku80, DNA-PKcs, and ATM in 41 cases of cervical carcinoma and 15 cases of CIN. Results 　The positive percentages of Ku80, DNA-PKcs and ATM were 70. 7 %, 68. 3 % and 19. 5 % respectively in cervical carcinoma, and were 80. 0 %, 73. 3 % and 33. 3 % respectively in CIN ; the expression of three proteins in CIN were all higher than that in cervical carcinoma, but the differences has no statistical significance ( P > 0. 05) . The expression of these proteins was not associated with the age of patients, pathology, differentiation, and clinical stage ( P > 0. 05) . In all 56 cases, Ku80 was positively correlated with DNA2PKcs ( r = 0. 583, P = 0. 000) and ATM ( r = 0. 274, P = 0. 041) . Conclusion 　Three proteins might not play the major role in the neoplasia and tumor progression of cervical carcinoma ; Ku80 had closely relationship with DNA2PKcs and ATM in cervical carcinoma and CIN.

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