Abstract: Objective 　To evaluate chemotherapy efficacy and toxicity of Cisplatin chronotherapy and non chronotherapy combined with HCPT on advanced non-small cell lung cancer. Methods 　Fifty-nine cases of stage Ⅲb～ Ⅳ advanced non-small cell lung cancer were random divided into two groups, one group treated with Cisplatin chronotherapy and HCPT regimen, the other t reated with Cisplatin non chronotherapy and HCPT regimen. The dose of two drugs was the same in two groups Cisplatin 14 mg/ (m²·d), HCPT 6 mg/ (m²·d) , two drugs were int ravenous drip d1～5 ; the Cisplatin chronotherapy group, Cisplatin was given on at eighteen o′clock, HCPT was given in routine (at half past nine), the Cisplatin non chronotherapy group, the Cisplatin and HCPT was given in routine, the t reatment was recycled every 21 days, the therapeutic effect and side-effect s was evaluated over 2 cycles. Results 　In chronotherapy group, PR was 13 cases, SD was 13 cases, PD was 4 cases, CR + PR was 43. 3 %, in non chronotherapy group, PR was 9 cases, SD was 17 cases, PD was 3 cases, CR + PR was 31. 0 %, ( P = 0. 051), In chronotherapy group, median survival time was 6. 62 months, 12 months survival rate was 30. 4 %(9/30), 18 months survival rate was 13. 3 %(4/ 30), in non chronotherapy group, median survival time was 5. 06 months, 12 months survival rate was 24. 1 %(7/ 29), 18 months survival rate was 3. 4 %(1/ 29), The side effects was the same in two groups. Conclusion 　Cisplatin chronotherapy combined with HCPT is regarded as a valuable regimen for advanced non-small cell lung cancer, but it needs to be further studied and evaluated in randomized trial.