Abstract: Objective 　To investigate the effect of the nuclear factor-kappa B (NF-κB) subunit p65 antisense oligonucleotide (ASODN) on the expression of intercellular adhesion molecule-1 ( ICAM-1) and invasive ability of pancreatic carcinoma cells. Methods 　p65 ASODN were designed and const ructed and were transfected into human pancreatic cancer cell line PANC-1. The rate of t ransfection was detected by flow cytometry and fluorescence microscope. RT-PCR was used to detect the expression levels of p65 and ICAM-1 mRNA. MTT assay and invasive tests were used to detect the proliferation and invasive ability of PANC-1 cells. Results 　p65 ASODN could be successfully transfected into PANC-1 cells. After transfection, the levels of p65 and ICAM-1 mRNA expression, cell proliferation and the invasive ability of PANC-1 cells were reduced ( P < 0. 01) . Conclusion 　Transfection of p65 ASODN could effectively inhibit the expression of p65 mRNA and reduce invasive and metastasis ability of PANC-1 cells. The down-regulation of ICAM-1 may play an important role in the inhibitive effect of p65 ASODN.