人肝癌细胞放射敏感性体外研究

Study of Radio sensitivity of Hepatocar cinoma Cell Line sin Vitro

摘要: 目的　用体外实验方法探讨人肝细胞癌的放射敏感性。方法　三株人肝癌细胞HepG2、SMMC 74 0 2、SMMC 772 1体外培养, 应用集落形成法和MTT法测定 6MVX线不同剂量照射后细胞的存活率, 计算三株细胞放射敏感性参数。结果　HepG2的D0 为 1.5, SF2 为 0 .4 5 ;74 0 2的D0 为 1.6, SF2 为 0 .6 3;770 1的D0 为 1.8,SF2 为 0 .79。MTT法与集落形成法相关性较好, r =0 .95 8(P <0 .0 5 )。结论　HepG2具有较高的放射敏感性, 74 0 2和 772 1的放射敏感性较低 ;MTT法可替代集落形成法作细胞放射敏感性的快速测定。

Abstract: Objective To evaluate the radiosensitivity of hepatocarcinoma cells in vitro. Methods Using cell culture of human being hepatocarcinoma cell lines- HepG2, SMMC-7402 and SMMC-7721, we measured their surviving fraction after being irradiated by 6MV X-ray with doses from 0～10Gy by means of clonogenic assay and MTT. Radiosensitivity indexes have been calculated. Results The D 0 of HepG2 is 1.5,SF 20.45±0.05Gy.The D 0 of 7402 is 1.6, SF 20.63±0.05Gy.The D 0 of 7721 is 1.8, SF 2 0.79±0.07Gy.There is a good...