β-胡萝卜素对人体乳腺癌MCF-7细胞的细胞毒性研究

Anti-Tumor Effects of β-Carotine on MCF-7 Human Breast Cancer Cells in Vitro

摘要: 目的　研究 β 胡萝卜素对人体乳腺癌MCF 7细胞毒杀作用。方法　应用MTT比色法, 在试管内研究 β CA对MMTOD值的影响, 检测 β CA对MCF 7人乳腺细胞的毒杀作用。结果　不同浓度的 β CA对MCF 7癌细胞均有杀伤作用。特别是 0 .5、0 .1、0 .0 5mg/ml组, 其对癌细胞的杀伤率分别达到 99%、88.19%和 5 6 .35 %, 有极显著的统计学差异 (P <0 .0 1)。结论　β CA在试管内对MCF 7乳腺癌细胞有直接的毒杀作用, 作用机理可能与其直接调控癌基因和抗癌基因的表达有关, 最终可导致癌细胞的死亡。

Abstract: Objective To study the cytotoxic effects of β carotine on MCF-7 human breast cancer cells in vitro. Methods By using modified MTT colorimetry the cytotoxity of β CA on MCF-7 human breast cancer cells was monitored. Results Allconcentrations of β CA had cytotoxic effects on MCF-7 tumor cells especially the killer rate of groups of 0.5,0.1,0.05mg/ml reached 99%,88.19%, and 56.35% respectively.There were statistically striking differences( P<0.01 ). Conclusion β CA had a direct cytotoxic effects on MC...