兔VX2肝癌介入治疗后早期发生凋亡的流式细胞术检测
Flow Cytometry Research on Tumor Cell Apoptosis of Rabbit VX2 Liver Implanted Tumor in Earlier Period After Interventional Treatment
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摘要: 目的研究经肝动脉插管灌注碘油抗癌药乳液化疗栓塞和单纯灌注化疗后的早期兔肝VX2肿瘤发生凋亡。方法27只新西兰大白兔瘤组织块种植肝VX2肿瘤,A组(n=9)经肝动脉灌注碘油抗癌药乳液,B组(n=9)肝动脉灌注化疗,C组(n=9)肝动脉灌注生理盐水,每组分别在处理后第24h、72h、120h处死实验兔各3只,采用FITC-AnnexinV/PI流式细胞术分析介入处理后早期的肿瘤细胞凋亡。结果肿瘤边缘部的凋亡早期细胞百分率(PapE)与凋亡晚期细胞百分率(PapL)及峰值时间点分别为:A组(9.48±4.58)%、(33.46±8.81)%、24h、72h,B组(4.46±1.47)%、(11.65±3.51)%、24h、24h,C组(2.50±1.39)%、(5.99±1.22)%。肿瘤中心部的PapE、PapL与峰值时间点分别为:A组(16.48±6.32)%、(43.56±18.51)%、24h、72h,B组(7.50±3.10)%、(18.53±14.04)%、24h、24h,C组(5.61±1.92)%、(14.63±12.23)%。结论Lp-THACE诱导肝癌肿瘤细胞早期大量凋亡是其有效的主要作用机制,...Abstract: Objective To research the tumor cell apoptosis with flow cytometry for rabbit VX2 liver implanted tumor in earlier period after Lp-THACE and THAI. Methods 27 New Zealand white rabbits were implanted VX2 tumor at left middle lobe of the liver,were divided into three groups which treated by transhepatic artery catheterrizeation:Group A(n=9),Lp-THACE; Group B(n=9),THAI; Group C(n=9) as control.At 24h,72h,and 120h, three rabbits in each group were executed respectively. FITC-AnnexinV/PI were used for measuring ...
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