人肝癌细胞感染蓝舌病毒HbC_3株超微结构的动态观察

Dynamic Observation Microstructure of the Human Hepatic Carcinoma Cells Infected Bluetongue Virus HbC_3 Strain

摘要: 目的探讨蓝舌病毒靶向抗肿瘤的细胞生物学机制。方法利用透射电镜观察蓝舌病毒HbC3株感染人肝癌细胞Hep-3B的形态发生学以及该病毒引起的细胞的病理改变。结果BTV-HbC3以受体介导的胞饮作用穿入细胞,溶酶体水解病毒外衣壳,使之成为亚病毒粒子,胞浆内有病毒包涵体及未装配成熟的亚病毒颗粒。随后亚病毒颗粒装配上外层蛋白结构,形成成熟的病毒粒子。病毒感染细胞12~18h时,细胞以挤出的方式释放病毒并达到高峰。18~48h时,病毒进入超感染期,大量细胞发生病变,出现细胞凋亡和溶解。结论一旦蓝舌病毒感染Hep-3B肿瘤细胞即可在细胞内增殖并诱导该肿瘤细胞进入凋亡,死亡的肿瘤细胞释放出的病毒粒子并再次感染其他肿瘤细胞,直至将全部肿瘤细胞杀灭,其溶瘤方式为链式反应。

Abstract: Objective The cytobiological mechanism of BTV-HbC_ 3 killed the tumor cells were investigated in this study. Methods The morphogenesis and the pathological changes of the Hep-3B cells infected Bluetongue virus were studied by transmission electron microscope. Results BTV-HbC_ 3 were penetrated into cell with pinocytpsis. Viral outside capsid were hydrolated with hydrolase in the lysosome, which become subvirual particles. The viral inclubodies and subviral particles without outer layer proteins were observe...