抗VEGF抗体及抗KDR抗体对裸鼠胃癌细胞增殖和凋亡的影响

The Effect of Anti-vascular Endothelial Growth Factor and Receptor Antibody on the Proliferation Activity and Apoptosis of Human Gastric Cancer

摘要: 目的探讨抗VEGF抗体及抗KDR抗体对胃癌细胞增殖和凋亡的影响。方法建立胃癌原位种植模型;7天后注射抗VEGF抗体、抗KDR抗体及二者联合注射,连续给药8周,每周2次,第10周末处死动物,用免疫组化法、RT-PCR法检测移植瘤组织中VEGF蛋白和mRNA表达情况;FCM检测肿瘤细胞增殖及凋亡情况。结果与对照组相比,抗VEGF抗体和抗KDR抗体组肿瘤组织中VEGF蛋白的表达降低,而VEGFmRNA表达无显著变化;肿瘤细胞发生不同程度的凋亡,凋亡率明显增高,G0/G1期细胞明显增多,S期细胞明显减少,细胞增殖指数降低;两者合用时作用更为明显。结论通过中和VEGF抗体和封闭KDR受体抑制血管形成,可能为胃癌的抗血管生成治疗提供依据。

Abstract: Objective To study the inhibiting effect of anti-vascular endothelial growth factor and receptor antibody on growthmetastasis and apoptosis of gastric cancer. Methods The inhibitory effect of anti-VEGF antibody, anti-KDR antibody were investigated by means of an orthotopic xenotransplanted model of mice which had been randomly divided into 4 groups: ①control group, ②group receiving anti-VEGF antibody, ③group receiving anti-KDR antibody and ④group receiving anti-VEGF antibody and anti-KDR antibody. Anti-VEGF...