IFN-γ基因+874位点单核苷酸多态性与乳腺癌的相关性
Association of Single Nucleotide Polymorphism of Interferon-gamma Gene +874 Site and Breast Cancer
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摘要: 目的探讨IFN-γ基因+874位点单核苷酸多态性与乳腺癌的相关性。方法采用PCR技术对94例乳腺癌患者及96例正常女性IFN-γ基因+874位点A/T单核苷酸多态性(SNP)进行分析,将PCR产物进行克隆、测序。结果乳腺癌患者IFN-γ基因+874位点TT基因型频率为18.1%,显著高于正常人的8.3%,两者之间比较具有显著性差异(χ2=3.95,P=0.047);乳腺癌病例组T等位基因频率显著高于对照组(χ2=4.984,P=0.026)。进一步分析显示,乳腺癌病例组中不同年龄段基因型频率和等位基因频率相比,均无显著性差异。结论IFN-γ基因+874位点TT基因型可能与乳腺癌的发生相关,T等位基因可能为乳腺癌发生的遗传危险因素。Abstract: Objective To explore the relationship between the single nucleotide polymorphism of interferon-gamma gene +874 site and breast cancer. Methods Using PCR technique to analyze the single nucleotide polymorphism (SNP)of Interferon-gamma Gene +874 site A/T of 96 normal women and 94 female breast cancer patients, the PCR products were cloned and sequenced. Results The frequency of interferon-gamma gene +874 site TT genotype was significantly higher in patients with breast cancer than that in the healthy individuals(18. 1 % vs 8. 3 %, P < 0. 05) ; T allele f requency was higher in breast cancer group than in normal group . But the TT genotype had no significant correlation with the age in breast cancer patient s. Conclusion TT genotype of IFN-γ+ 874 site might have a relationship with generation of breast cancer.
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