TGF-β1对人肝癌细胞系凋亡的调控作用研究
Study on TGF-β1-Mediated Apoptosis of Human Hepatoma Cell Lines
-
摘要: 目的 本研究旨在明确人肝癌细胞系中TGF β1的作用及其与凋亡的关系。 方法 本研究选用了 3种含有不同 p5 3基因状态的人肝癌细胞系, 应用TUNEL技术对TGF β1诱导的肝癌细胞的凋亡进行了定量检测。结果 TGF β1仅能诱导HepG2细胞 (野生型p5 3)凋亡, 这提示凋亡与 p5 3基因的表达具有明确的联系。结论 HepG2细胞系比Huh 7和Hep3B系细胞更易发生TGF β1诱导的凋亡, TGF β1通过p5 3依赖性途径诱导肝癌细胞系发生凋亡Abstract: Objective The aim of the present study was to determine the relationship between the effect of TGF-β1 and apoptosis in human hepatoma cell lines. Methods Three human hepatic carcinoma cell lines, involving different status of the p53 gene respectively, were used in this study. TGF-β1-induced apoptosis in hepatic carcinoma cell lines was quantitated using TUNEL assay. Results Addition of TGF-β1 induced apoptosis only in HepG2 cells (wild type p53). It was indicated that there is positive correlation between...
下载:
