地塞米松对小鼠H_(22)肿瘤生长及血管内皮生长因子表达的影响
Effect of dexamethasone on tumor growth and vascular endothelial growth factor expression in mouse H_(22) model
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摘要: 目的 探讨地塞米松对小鼠H2 2 肿瘤生长及血管内皮生长因子 (VEGF)表达的影响。方法 BALB c小鼠皮下接种H2 2 肿瘤细胞, 腹腔注射地塞米松, 每天测肿瘤直径。用抗CD31单抗对肿瘤组织做免疫组化微血管计数。噻唑蓝法检测地塞米松对体外培养的H2 2 细胞和人脐静脉内皮细胞ECV 30 4增殖的影响。RT PCR法测定肿瘤组织和体外培养细胞的VEGFmRNA表达。结果 地塞米松可以显著抑制体内H2 2 肿瘤的生长, 给药组肿瘤体积与对照组比较P <0 .0 5。给药组微血管计数和VEGFmRNA表达比对照组显著减少。体外实验中, 随着地塞米松浓度的增加, ECV 30 4细胞的增殖率降低 ;H2 2 细胞中VEGFmR NA表达下降。结论 地塞米松可以显著抑制小鼠H2 2 肿瘤的生长。地塞米松对体内H2 2 肿瘤组织和体外H2 2 细胞的VEGFmRNA表达均有显著的抑制作用。地塞米松对肿瘤细胞VEGF表达的抑制作用可能是其抗H2 2 肿瘤及抗血管生成的一个重要原因。Abstract: Objective To observe the effect of dexamethasone on tumor growth and vascular endothelial growth factor (VEGF) expression in mouse H 22model. Methods H 22 cells were inoculated subcutaneous into BALB/c mice. Intraperitoneal injection of dexamethasone began from the day inoculation. Diameters of tumor were measured every day. Tumor microvessel count was performed by immunohistochemical staining with anti-CD31 monoclonal antibody. Proliferation of H 22 cells and ECV-304 cells (human umbilic...
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