大肠癌中Fas、FasL、bcl-2蛋白的表达及意义
Expression of Fas、FasL、bcl-2 protein in colorectal carcinomas
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摘要: 目的 探讨Fas、FasL、bcl 2在大肠癌中表达及意义。方法 用免疫组化S P法检测 15例大肠腺瘤、4 0例大肠腺癌及 15例大肠正常组织Fas、FasL、bcl 2蛋白表达, 以TUNEL法测定细胞凋亡。结果 腺瘤和腺癌凋亡指数高于正常组织, 腺瘤组高于腺癌组 (P <0 .0 5 )。在大肠正常组织、腺瘤、腺癌中Fas阳性率下降, FasL阳性率上升, 有显著性差异 (P <0 .0 5 )。腺瘤组和腺癌组bcl 2表达高于正常组 (P <0 .0 5 )。bcl 2阳性组FasL阳性率高于bcl 2阴性组 (P <0 .0 5 )。结论 瘤细胞Fas FasL表达异常使肿瘤逃脱机体自身免疫攻击, 促使肿瘤的发生、发展, 对预测大肠癌的预后有重要参考价值。bcl 2高表达是癌变过程中的早期事件, 有利肿瘤的早期诊断。Abstract: Objective To investigate the significance of Fas?FasL and bcl 2 in human colorectal carcinoma. Methods The expression of Fas?FasL and bcl 2 protein were detected by immunohistochemical method in paraffin sections of 15 cases of colorectal adenomas? 40 cases of colorectal carcinomas and 15 cases of normal colorectal mucous. The TdT mediated dUTP biotin nick and labeling(TUNEL) positive were counted in all cases as apoptosis index(AI). Results AI in colorectal carcinoma was higher than in normal t...
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