蝎毒抗癌多肽对人肝癌细胞株的作用

The effect of APBMV on human hepotoma cell line

摘要: 目的观察蝎毒抗癌多肽（ＡＰＢＭＶ）对人肝癌细胞ＳＭＭＣ－７７２１的作用。方法ＭＴＴ法、生长抑制实验和集落形成实验。结果ＡＰＢＭＶ对ＳＭＭＣ－７７２１细胞具较强毒性作用，ＩＣ５０为１１．３３μｇ／ｍｌ；ＡＰＢＭＶ可显著抑制细胞生长，剂量与时间效应关系明显。结论ＡＰＢＭＶ是东亚钳蝎蝎毒中有效低毒的抗肿瘤成分，对肝癌细胞有一定的毒性作用。

Abstract: Objective To obserne the effect of antineoplastic polypeptide from Buthus martensii venom (APBMV) on the human heptoma cells SMMC-7721. Methods Using the MTT colorimetric method, the growth-inhibiting test and the colony-formation inhibiting assay. Results The results revealed that APBMV had obvious cytotoxicity on SMMC-7721,1C50 was 11. 33μg/ml;APBMV could obviously inhibit the cellu- lar growth. There were obvious dose-response and timeresponse relationship. The IC50 we...