Celecoxib通过环氧合酶2和前列腺素E_2途径抑制胰腺癌JF-305细胞增殖
Celecoxib inhibits proliferation and induces apoptosis via cyclooxygenase-2 and PGE_2 pathway in human pancreatic carcinoma cell line JF-305
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摘要: 目的 研究Celecoxib对COX 2高表达人胰腺癌细胞JF 30 5生长和凋亡的影响及作用机制。方法 采用四唑氮蓝 (MTT)比色法检测细胞增殖, 流式细胞仪测定细胞周期和凋亡, 酶联免疫吸附试验 (ELISA)检测前列腺素E2 (PGE2 )含量。结果 Celecoxib可明显抑制JF 30 5细胞增殖和诱导其凋亡, 并且这种增殖抑制作用能被PGE2 拮抗。结论 Celecoxib通过COX 2和PGE2 途径抑制JF 30 5细胞生长和诱导其凋亡 ;Celecoxib可能是COX 2高表达胰腺肿瘤的一种有效的化学治疗和化学预防药物。Abstract: Objective To evaluate the effects and mechanisms of celecoxib in inducing proliferation inhibition and apoptosis on human pancreatic carcinoma cells. Methods The anti-proliferative effect was measured by using Methabenzthiazuron (MTT) assay. Cell cycle and apoptosis were analyzed by using flow cytometry. The prostaglandin E 2 (PGE 2) levels in the supernatant of cultured pancreatic carcinoma cells were quantitated by enzyme-linked immunoabsordent assay (ELISA). Results Celecoxib suppressed the productio...
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