Correlation between survivin Expression and Clinicopathological Factors in Colorectal Cancers
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摘要: 目的探讨结直肠癌survivin选择性剪接变体表达及其与临床病理参数之间的关系,揭示其在结直肠癌发生发展中的作用及临床意义。方法RT-PCR结合免疫组织化学技术,对survivin各种剪接变体在结直肠癌和癌旁组织中的表达进行了研究,同时还对survivin剪接变体survivin-2B及survivin-ΔEx3表达和临床病理资料作了相关性分析。结果结直肠癌组织和癌旁组织都表达survivin,并且得到免疫组织化学的证实;survivin-2B主要表达于癌旁组织,而survivin-ΔEx3则主要在癌组织中表达且与肿瘤转移相关。结论survivin不同变体表达之间的平衡关系可能对肿瘤的发生和发展起重要作用。
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关键词:
- survivin基因 /
- 剪接变体 /
- 结直肠癌 /
- RT-PCR
Abstract: Objective To investigate the expression of survivin variants and their relationship to each other as well as to clinic pathological factors in colorectal cancer. Methods survivin variant specific RT-PCR was developed to analysis their expression in 67 paired cancer and para-cancer tissues, and the expression of the wildtype survivin at the protein level was further verified by immunohistochemistry. Results All the cases tested expressed survivin mRNA，and immunohistochemistry obtained similar finding. The nonantiapoptostic survivin-2B was dominantly expressed in para-cancer tissues, whereas the antiapoptostic survivin-ΔEX3 was more frequently detected in cancer tissues. The expression of survivin-ΔEX3 mRNA was significantly associated with tumor metastasis. Conclusion The balance between antiapoptostic survivin iso-forms and nonantiapoptostic ones may play an important role in tumor genesis and tumor progression.-
Key words:
- survivin /
- Splice variants /
- Colorectal cancer /
- RT-PCR
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