伏马菌素B1对人结肠腺癌细胞培养倍增时间的影响

Fumonisin B1 May Reduced the Doubling Time of Cultured Cells

摘要: 目的　观测伏马菌素B1(FB1)对培养细胞倍增时间的影响，探讨FB1诱发肿瘤的可能机理。方法　利用荧光分光光度法测定原位溶解的人结肠腺癌培养细胞的DNA，以DNA总量的倍增作为培养细胞数目的倍增，通过比较对照组和FB1处理组DNA倍增的时间，判断出FB1对该细胞株细胞倍增时间的影响。结果　对照组结肠腺癌细胞的倍增时间在39～42小时，FB1处理组细胞的倍增时间为34～36小时。结论　伏马菌素B1可明显缩短人结肠腺癌细胞株的倍增时间。

Abstract: Objective To determine whether fumonisin B1(FB1) affec ts the doubling time of cell strain from cultured human colon adenocarcinoma(Cac o-2)，and to probe the possible mechanism that FB1 induced cancer. Methods The fluorescence spectrophotometry was used t o determine the contents of DNA from in situ solubilized cultured cells,the doubling of total DNA contents was used as the quantity doubling of cultured cells.After mat ched the total DNA contents of control groups with the treated groups by FB1,the effect of FB1 on the doubling time of Caco-2 cells was determined. Results The doubling time of control Caco-2 cells was about 39 to 42 hours.However,for the treated groups by FB1,the doubling time of Caco-2 ce lls was 34 to 36 hours. Conclusion Fumonisin B1 obviously reduced the doubling time of t he cells from cultured human colon adenocarcinoma.