钙载体A_(23187)快速诱导人外周血单核细胞成树突状细胞
Calcium Ionophore- treated Human Peripheral Blood Mononcytes Rapidly Acquire Activated Dendritic Cells
-
摘要: 目的 探讨钙载体 (calciumionophore, CI)A2 3 187对正常人外周血单核细胞来源的树突状细胞(Dendriticcell,DC)形态、表面分子表达及免疫功能的影响。方法 分离健康献血者外周血单核细胞, 分别加入 :重组人粒 /单集落刺激因子 (rhGM CSF) 10 0ng/ml、rhGM CSF 10 0ng/ml +IL 4 5 0ng/ml、rhGM CSF +A2 3 187(钙载体, CI)各 10 0ng/ml。体外培养 4 0h后, 于光镜及电镜下观察细胞的形态、流式细胞仪检测细胞的表面标志、MTT比色法检测上述细胞对同种异体T细胞的刺激增殖作用。结果 外周血单核细胞在GM CSF +A2 3 187各 10 0ng/ml的条件下培养 4 0h, 就可看到典型的DC形态, 其表面单核细胞的特征性标志CD14分子表达减少, HLA DR、CD80、CD86及CD83分子的表达均明显增高, 且具有明显刺激同种异体T细胞增殖的能力。结论 外周血单核细胞在钙载体A2 3 187及GM CSF的共同作用下, 4 0h就能获得成熟的DCAbstract: Objective To explore the effect of A 23187 (calcium ionophore, CI) on dendritic cells (DC) from healthy human peripheral blood monocytes. Methods Peripheral blood monocytes from healthy donors were treated with rhGM CSF of 100 ng/ml、rhGM CSF of 100ng/ml plus IL 4 of 50ng/ml and rhGM CSF plus A 23187 100ng/ml each, respectively. After cumtivation of 40 hours, change of cellular morphology was observed under light microscopy and electron microscopy. Surface markers on DCs were analyzed by flow...
下载:
