错配修复基因hMLH1、hMSH2及PCNA在肺癌组织中的表达及意义
Expression of Human Mismatch Repair Gene(hMLH1/hMSH2) and Proliferating Cell Nuclear Antigen(PCNA) in Lung Cancer and Its Significance
-
摘要: 目的 探讨人类错配修复基因hMLH1、hMSH2及增殖细胞核抗原PCNA在肺癌组织中的表达及意义。方法 运用免疫组织化学S P法对 5 6例肺癌组织中hMLH1、hMSH2及PCNA的表达进行检测。结果 5 6例肺癌组织中hMLH1的阳性表达率为 35 %, hMSH2阳性表达率为 2 8.6 %, 分化程度高者阳性率显著高于分化程度低者 (P <0 .0 1), 有淋巴结转移者hMLH1及hMSH2阳性率低于无淋巴结转移者 (P <0 .0 5 ), 不同病理组织学类型之间hMLH1及hMSH2表达无显著差别 (P >0 .0 5 ) ;5 6例肺癌组织中分化程度低者PCNA标记指数高于分化程度高者 (P <0 .0 1), 有淋巴结转移者PCNA标记指数高于无淋巴结转移者 (P <0 .0 5 ), hMLH1及hMSH2阴性表达者的PCNA标记指数明显高于hMLH1及hMSH2阳性表达者 (P <0 .0 1), 不同病理组织学类型之间PCNA标记指数无显著差别 (P >0 .0 5 )。结论 hMLH1及hMSH2基因的缺陷及PCNA的表达与肺癌的发生发展过程并与分化程度及有否淋巴结转移有关Abstract: Objective To investigate the expression of human mismatches repair gene (hMLH1/hMSH2) and proliferating cell nuclear antigen (PCNA) in lung cancer and its significance. Methods Expression of hMLH1/hMSH2 and PCNA in 56 cases with lung cancer was examined by S P immunohistochemical staining. Results In 56 cases with lung cancer,the positive expression rate of hMLH1 and hMSH2 was 35% and 28.6% respectively.The positive rate of hMLH1/hMSH2 expression in the patients with high and middle differential grad...
下载:
