白藜芦醇抑制人脑胶质瘤细胞生长及诱导凋亡比较

doi:10.3971/j.issn.1000-8578.2471

白藜芦醇抑制人脑胶质瘤细胞生长及诱导凋亡比较

Comparison Study on Resveratrol Suppressing Human Glioma Cells Growth and Inducing Apoptosis

摘要

摘要: 目的探讨白藜芦醇（Resveratrol，Res）体外抑制U251、U87和SHG-44脑胶质瘤细胞生长及诱导凋亡的不同作用比较，验证Re8确可导致U251细胞凋亡。方法 MTT比色法测量不同剂量的Res作用U251、U87和SHG-44胶质瘤细胞24h后对增殖的影响及不同剂量的Res作用U251、U87和SHG-44细胞6h、24h、48h后的影响。流式细胞仪用Annexin V-FITC和PI双染检测U251、U87和SHG-44细胞凋亡率。HE染色、Hoechst 33342荧光染色、透射电镜（TEM）观察U251细胞形态改变，流式细胞仪测定U251细胞的细胞周期改变。结果 Res明显抑制U251、U87和SHG-44细胞的生长和增殖（P〈0．01）且对U87细胞的抑制作用最强，U251和SHG-44细胞次之；对U251细胞的抑制作用呈浓度及时间依赖性反应；Re8所致的U251、U87和SHG-44胶质瘤细胞凋亡为浓度依赖关系，且对U87细胞的凋亡作用强于U251和SHG-44细胞。U251凋亡细胞的细胞周期主要发生G，期阻滞。结论 Res对U251、U87和SHG-44细胞生长抑制及凋亡作用以U87细胞更明显，对U251细胞的抑制作用呈浓度及时间依赖性反应并引起了其细胞周期改变。

Abstract: Objective 　To explore the different cont ribution of resveratrol (Res) to the proliferation and apoptosis of glioma U251, U87 and SHG-44 cells in vitro, verify the apoptosis of U251 cell caused by Res. Methods 　MTT assay was used to measure it' s effect s on the proliferation of U251, U87 and SHG-44 cells cultured with different concent rations of Res for 24h and it' s effect s on the proliferation of U251, U87 and SHG-44 cells for 6h, 24h and 48h. The Annexin VFITC and PI assay by flow cytometer were applied to detect the apoptosis of U251, U87 and SHG-44 cells. HE staining, Hoechst 33342 fluorescence staining, transmission elect ron microscope ( TEM) was used to observe the changes of U251 cellular morphous. The cell cycle change of U251 cells was detected by FCM. Results 　Resobviously suppressed the proliferation of U251, U87 and SHG-44 giloma cells ( P < 0. 01), and the suppressive effect concent ration-dependent manner, and the apoptosis rate of U87 cell was higher than that of U251 and SHG-44 cells. Rescaused U251 cell cycle mainly with G arrest . Conclusion 　Res caused U87 cells more significant cytotoxicity and apoptosis than U251 and SHG244 cells, Res induced U251 cell cycle mainly with G arrest .

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