转PTEN 基因对人结肠癌细胞的抑制作用
Inhibition of Transfected PTEN on Human Colon Cancer
-
摘要: 目的 探讨PTEN基因转染对结肠癌LoVo细胞的抑制作用. 方法 将人野生型及突变型PTEN cDNA通过脂质体导入结肠癌细胞LoVo中,并用RT-PCR、Western blot、流式细胞术检测细胞的PTEN mRNA和蛋白质表达.用流式细胞仪、DNA梯形带和MTT法分析5-Fu对转染前后LoVo细胞抑制周期和诱导凋亡的能力. 结果 转染野生型PTEN基因可明显增强结肠癌LoVo细胞的PTEN表达,5-Fu对转染后的LoVo细胞抑制细胞周期和诱导凋亡的作用明显增强.突变型在结肠癌细胞LoVo中有表达但无明显抑制作用. 结论 转染的PTEN基因可显著上调结肠癌LoVo细胞的PTEN表达,促进细胞凋亡,联合应用PTEN基因转染和5-Fu可获得协同的细胞毒作用.Abstract: Objective To study the inhibitory effect of transfected PTEN on LoVo cells. Methods The human PTEN cDNA was t ransferred into LoVo cells via lipofectin and PTEN mRNA levels and its expression were analysized by Western blot and flow cytometry. Before or af ter t ransfection, the activities of 5-Fu in inhibiting cell proliferation and inducing apoptosis were measured by flow cytometry, DNA bands and MTT. Results PTEN transfection significantly up-regulated PTEN expression in LoVo cells. 5-Fu inhibited cell proliferation and induced apoptosis in t ransfected LoVo cell. Conclusion Transfected PTEN remarkably up-regulated PTEN expression in LoVo cells and promoted the apoptosis. PTEN transfection associated with 5-Fu t reatment had cooperatively cytotoxic effect .
下载:
